In this study, Professor Li Wei's research group turned its attention to the emerging field of drug targeted delivery - the use of cell membranes of endogenous cells to coat drug carriers. This novel delivery system is intended to impart a specific function to the endogenous cell membrane of the drug carrier and to extend the circulation time of the drug carrier in vivo. When constructing this drug-targeted delivery system, researchers face two major questions: whether the endogenous cell membrane can effectively coat the nanocarriers and whether they can be coated in the right direction. In order to solve the above problems, Professor Li Wei's research group proposed a "molecular affinity" strategy, based on the specific interaction between the intracellular region of the important transmembrane receptor on the cell membrane and its corresponding polypeptide ligand, to guide the cell membrane efficiently, Properly coated drug carrier. In view of the interaction between the pathogenic fungus and the host red blood cells, the research team selected the red blood cell major transmembrane receptor band 3 as the "hand grip", first designed the peptide ligand specifically binding to the band 3 intracellular region, and confirmed it with OpenSPR. The affinity of the polypeptide ligand to band 3. In addition to the determination of affinity, the researchers also completed a competitive binding assay on OpenSPR, further confirming the effective binding of the peptide ligand to band 3. After the polypeptide ligand is inserted into the liposome, the erythrocyte membrane coats the liposome through interaction between the intracellular band 3 intracellular region and the liposome surface polypeptide. It has been experimentally verified that the liposome effectively inherits the immunocompatibility of red blood cells and the long-circulating properties in vivo. At the same time, the liposome can also achieve targeted drug delivery of pathogenic fungi due to the property of the pathogenic fungus to capture the uptake of iron ions by red blood cells. In this study, the erythrocyte membrane-coated biomimetic liposome was constructed by the "molecular affinity" strategy, which provided a new idea for the construction of endogenous cell membrane coating drug delivery system, and can be extended to various biofilm-related nanomaterials. Nano drug research field. The next generation of SPR technology that breaks through the zero technology threshold Purui Maddy (Beijing) Laboratory Technology Co., Ltd. Address: Room 401, International Business Incubation Center, No. 9 Kexing Road, Fengtai District, Beijing phone fax mailbox: Stretcher Base,Aluminum Alloy Stretcher Base,Stretcher Platform Base,Ambulance Stretcher Base Medton Medical , https://www.medtonmedical.com
OpenSPR assists in biomimetic delivery system research
Professor Li Wei from the School of Pharmacy of Southwest University is dedicated to the design, screening and optimization of biologically active functional peptides, focusing on the targeted delivery of peptide-mediated drugs. Following the publication of a high-level research paper on targeted delivery of anti-fungal infection delivery systems via oral route at Nano Letters (IF:12.08) in October 2018 ( Nano Letters Newsletter---OpenSPR Molecular Interactions Help Oral Pneumonia Nanotargets To the drug research ), recently, Professor Li Wei's research group has made new breakthroughs in the field of drug targeted delivery, published in the internationally renowned journal ACS Nano (IF: 13.709) entitled "Oriented Assembly of Cell-Mimicking Nanoparticles via a Molecular Affinity Strategy Research paper for Targeted Drug Delivery. The paper was selected as the cover paper for ACS NANO in May. Both studies used Nicoya's OpenSPR to obtain key data on the affinity of the polypeptide ligand to the target molecule.